Western Blot: Rffl Antibody (3A4) [H00117584-M01] - Analysis of RFFL expression in transfected 293T cell line by RFFL monoclonal antibody (M01), clone 3A4.Lane 1: RFFL transfected lysate(36.6 KDa).Lane 2: Non-transfected lysate.
Western Blot: Rffl Antibody (3A4) [H00117584-M01] - Analysis of RFFL over-expressed 293 cell line, cotransfected with RFFL Validated Chimera RNAi ( Cat # H00117584-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RFFL monoclonal antibody (M01), clone 3A4 (Cat # H00117584-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: Rffl Antibody (3A4) [H00117584-M01] - Detection limit for recombinant GST tagged RFFL is 0.03 ng/ml as a capture antibody.