Western blot analysis of extracts from NCI-H3255 cells, untreated (-) or λ phosphatase-treated (+), using Phospho-TTF-1 (Ser327) Rabbit Antibody (upper), Thyroid Transcription Factor 1 (D2E8) Rabbit mAb #12373 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunoprecipitation of phospho-TTF-1 (Ser327) from NCI-H3255 cell extracts using Normal Rabbit IgG #2729 (lane 2) or Phospho-TTF-1 (Ser327) Antibody (lane 3). Lane 1 is 10% input. Western blot analysis performed using Phospho-TTF-1 (Ser327) Antibody. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody.