Western blot analysis of extracts from SH-SY5Y cells, untreated or treated with AICAR #9944 (2 mM for 30 minutes), using Phospho-Tuberin/TSC2 (Ser1387) Antibody (upper) and Tuberin/TSC2 (D93F12) XP ® Rabbit mAb #4308 (lower).
Western blot analysis of extracts from SH-SY5Y cells, treated with AICAR #9944 (2 mM for 30 minutes), using Phospho-Tuberin/TSC2 (Ser1387) Antibody. The phospho-specificity of the antibody was verified by treating the membrane with (+) or without (-) calf intestinal phosphatase (CIP) after western transfer.