TSC2

Gene Symbol TSC2
Entrez Gene 7249
Alt Symbol LAM, PPP1R160, TSC4
Species Human
Gene Type protein-coding
Description tuberous sclerosis 2
Other Description protein phosphatase 1, regulatory subunit 160|tuberin|tuberous sclerosis 2 protein
Swissprots B7Z2B8 B4DRN2 P49815 A7E2E2 B4DIQ7 B4DIL8 O75275 Q8TAZ1 Q4LE71 C9J378
Accessions AAB41564 AAC34210 BAA32694 EAW85556 EAW85557 EAW85558 EAW85559 EAW85560 P49815 AB210000 BAE06082 AK022401 AK094152 BAG52831 AK125096 BAG54139 AK294548 BAH11804 AK295672 BAG58530 AK295728 BAG58569 AK299343 BAG61344 BC025364 AAH25364 BC046929 AAH46929 BC150300 AAI50301 BF194994 BM966762 BX647816 CAI46010 DA316794 DB288276 KJ534954 AHW56594 KJ534955 AHW56595 KJ534956 AHW56596 KJ534957 AHW56597 KJ534958 AHW56598 KJ534982 AHW56622 KJ534983 AHW56623 KJ534984 AHW56624 KJ534985 AHW56625 KJ535011 AHW56650 KJ535022 AHW56661 KJ535038 AHW56677 KJ535039 AHW56678 KJ535051 AHW56690 KJ535091 AHW56730 KJ535094 AHW56733 S82474 AAB37376 X75621 CAA53287 XM_005255529 XP_005255586 XM_005255531 XP_005255588 XM_011522636 XP_011520938 XM_011522637 XP_011520939 XM_011522638 XP_011520940 XM_011522639 XP_011520941 XM_011522640 XP_011520942 XM_011522641 XP_011520943 NM_000548 NP_000539 NM_001077183 NP_001070651 NM_001114382 NP_001107854 NM_021055 NM_021056
Function In complex with TSC1, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase- activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. Stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors. {ECO:0000269|PubMed:12271141, ECO:0000269|PubMed:15340059, ECO:0000269|PubMed:16707451}.
Subcellular Location Cytoplasm. Membrane; Peripheral membrane protein. Note=At steady state found in association with membranes.
Tissue Specificity Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta.
Top Pathways p53 signaling pathway, Insulin signaling pathway, PI3K-Akt signaling pathway, AMPK signaling pathway, Choline metabolism in cancer

Phospho-Tuberin/TSC2 (Thr1462) Antibody - 3611 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from NIH/3T3 cells, untreated, PDGF-treated (50 ng/ml), PDGF and wortmannin-treated (200 nM) or PDGF and rapamycin-treated (5 nM), and MCF-7 cells, untreated or IGF-1 treated for the indicated times, using Phospho-Tuberin/TSC2 (Thr1462) Antibody (upper) or Tuberin/TSC2 Antibody #3612 (lower).

Phospho-Tuberin/TSC2 (Tyr1571) Antibody - 3614 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from NIH/3T3 cells, untreated, PDGF-treated, PDGF and wortmannin-treated or PDGF and rapamycin-treated, using Phospho-Tuberin/TSC2 (Tyr1571) Antibody (top), Phospho-Tuberin/TSC2 (Thr1462) Antibody #3611 (middle) or Tuberin/TSC2 Antibody #3612 (bottom).

Phospho-Tuberin/TSC2 (Ser1387) Antibody - 5584 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from SH-SY5Y cells, untreated or treated with AICAR #9944 (2 mM for 30 minutes), using Phospho-Tuberin/TSC2 (Ser1387) Antibody (upper) and Tuberin/TSC2 (D93F12) XP ® Rabbit mAb #4308 (lower).

Phospho-Tuberin/TSC2 (Thr1462) (5B12) Rabbit mAb - 3617 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 5B12
Western blot analysis of extracts from l phosphatase-treated or PDGF treated NIH/3T3 cells, using Phospho- Tuberin/TSC2 (Thr1462) (5B12) Rabbit mAb (upper) or Tuberin Antibody (lower).

Phospho-Tuberin/TSC2 (Ser1254) Antibody - 3616 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from CAD and PC12 cells, using Phospho-Tuberin/TSC2 (Ser1254) Antibody. The phospho-specificity of the antibody was verified by treating the membrane with (+) or without (-) calf intestinal phosphatase (CIP) after Western transfer.

Phospho-Tuberin/TSC2 (Ser939) Antibody - 3615 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from NIH/3T3 cells, untreated, PDGF-treated, and PDGF and wortmannin-treated or PDGF and rapamycin-treated, using Phospho-Tuberin/TSC2 (Ser939) Antibody (top), Phospho-Tuberin/TSC2 (Thr1462) Antibody #3611 (middle) or Tuberin/TSC2 Antibody #3612 (bottom).

Tuberin/TSC2 (D93F12) XP ® Rabbit mAb - 4308 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D93F12
Western blot analysis of extracts from SH-SY5Y cells and rat brain using Tuberin/TSC2 (D93F12) XP ® Rabbit mAb.

Tuberin/TSC2 (D57A9) Rabbit mAb - 3990 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D57A9
Western blot analysis of extracts from SH-SY5Y and C6 cells, and from wild-type (wt) and TSC2-deficient (-/-) mouse embryonic fibroblasts (MEF), using Tuberin/TSC2 (D57A9) Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower).

Tuberin/TSC2 (28A7) Rabbit mAb - 3635 from Cell Signaling Technology

Prices $256.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 28A7
Western blot analysis of extracts from control SH-SY5Y, differentiated SH-SY5Y, PC3 and LNCaP cells, using Tuberin/TSC2 (28A7) Rabbit mAb.

Tuberin/TSC2 Antibody - 3612 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from NIH/3T3 cells, untreated, PDGF-treated (50 ng/ml), PDGF and wortmannin-treated (200 nM) or PDGF and rapamycin-treated (5 nM), and MCF-7 cells, untreated or IGF-1 treated for the indicated times, using Phospho-Tuberin/TSC2 (Thr1462) Antibody #3611 (upper) or Tuberin/TSC2 Antibody (lower).

tuberin (B-5) - sc-271314 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
tuberin (B-5): sc-271314. Western blot analysis of tuberin expression in Jurkat whole cell lysate.

tuberin (C-20) - sc-893 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
tuberin (C-20): sc-893. Immunofluorescence staining of methanol-fixed U-87 MG cells showing cytoplasmic localization.

tuberin (E-9) - sc-365103 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
tuberin (E-9): sc-365103. Western blot analysis of human recombinant tuberin fusion protein.

tuberin (H-300) - sc-13012 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
tuberin (H-300): sc-13012. Western blot analysis of tuberin expression in PDGF-treated 3611-RF whole cell lysate.

tuberin (N-19) - sc-892 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
tuberin (N-19): sc-892. Western blot analysis of human recombinant tuberin fusion protein.

p-tuberin (325.Ser 1798) - sc-293149 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of tuberin phosphorylation in untreated (A,D), PMA/Calyculin A treated (B,E) and PMA/Calyculin A and lambda protein phosphatase (sc-200312A) treated (C,F) Jurkat whole cell lysates. Antibodies tested include p-tuberin (325.Ser 1798): sc-293149 (A,B,C) and tuberin (B-5): sc-271314 (D,E,F).

p-tuberin (Ser 939) - sc-32838 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

p-tuberin (Thr 1462) - sc-32839 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-Tuberin (phospho S1254) antibody - ab138369 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin embedded Human prostate carcinoma tissue labelling ab138369 at 1/50 dilution.

Anti-Tuberin (phospho S1254) antibody [EPR5682] - ab133454 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5682
All lanes : Anti-Tuberin (phospho S1254) antibody [EPR5682] (ab133454) at 1/10000 dilutionLane 1 : SH-SY5Y cell lysateLane 2 : SH-SY5Y cell lysate treated with Okadaic Acid and Calyculin ALysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit IgG at 1/2000 dilution

Anti-Tuberin (phospho S664) antibody - ab116247 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ab116247, at a 1/50 dilution, staining Tuberin in Formalin-fixed, Paraffin-embedded Human skin tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-Tuberin (phospho S664) antibody [EPR8202] - ab133465 from Abcam

Prices $381.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8202
All lanes : Anti-Tuberin (phospho S664) antibody [EPR8202] (ab133465) at 1/1000 dilutionLane 1 : SH SY5Y cell lysatesLane 2 : SH SY5Y cell lysates treated with Okadaic acid and Calyculin ALysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-Tuberin (phospho S939) antibody - ab59269 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab59269 at a dilution of 1/50. Left hand image - without immunising peptide; right hand image - with immunising peptide.

Anti-Tuberin (phospho S939) antibody [EP1062Y] - ab52962 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1062Y
All lanes : Anti-Tuberin (phospho S939) antibody [EP1062Y] (ab52962) at 1/10000 dilutionLane 1 : 3T3 cell lysate untreatedLane 2 : 3T3 cell lysate treated with insulinLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP at 1/2000 dilution

Anti-Tuberin (phospho T1462) antibody - ab59274 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab59274 at a dilution of 1/50, in the presence (right) or absence (left) of immunising peptide.
Search more