Western Blot: ARHGEF6 Antibody (2E5) [H00009459-M02] - Analysis of ARHGEF6 expression in transfected 293T cell line by ARHGEF6 monoclonal antibody (M02), clone 2E5.Lane 1: ARHGEF6 transfected lysate (Predicted MW: 87.5 KDa).Lane 2: Non-transfected lysate.
Proximity Ligation Assay: ARHGEF6 Antibody (2E5) [H00009459-M02] - Analysis of protein-protein interactions between CDC42 and ARHGEF6. HeLa cells were stained with anti-CDC42 rabbit purified polyclonal 1:1200 and anti-ARHGEF6 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Immunoprecipitation: ARHGEF6 Antibody (2E5) [H00009459-M02] - Analysis of ARHGEF6 transfected lysate using anti-ARHGEF6 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ARHGEF6 monoclonal antibody.
Sandwich ELISA: ARHGEF6 Antibody (2E5) [H00009459-M02] - Detection limit for recombinant GST tagged ARHGEF6 is approximately 0.03ng/ml as a capture antibody.