Immunoprecipitation: CBX6 Antibody [NBP1-28686] - Detection of Human CBX6 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBX6 antibody NBP1-28686 used for IP at 10 mcg/mg lysate. CBX6 was also immunoprecipitated by rabbit anti-CBX6 antibody A302-283A, which recognizes an upstream epitope. For blotting immunoprecipitated CBX6, A302-283A was used at 1 mcg/ml, and the ReliaBLOT (Copyright) Reagents and Procedures were incorporated. Detection: Chemiluminescence with an exposure time of 30 seconds.