Western Blot: KIDINS220 Antibody [NBP1-50035] - Detection of Human ARMS by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ARMS antibod used for IP at 6 mcg/mg lysate. ARMS was also immunoprecipitated by rabbit anti-ARMS antibody which recognizes an upstream epitope. For blotting immunoprecipitated ARMS was used at 1 mcg/ml, and the ReliaBLOT Reagents and Procedures were incorporated. Detection: Chemiluminescence with an exposure time of 30 seconds.