![Western Blot: IBTK Antibody [NBP1-50033] - Detection of Human IBtk by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-IBtk antibody used for WB at 0.1 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. IBtk was also immunoprecipitated by rabbit anti-IBtk antibody BL10880, which recognizes an upstream epitope. For blotting immunoprecipitated IBtk, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_10/12327_IBTK-Antibody-Western-Blot-NBP1-50033-img0004.jpg)
Western Blot: IBTK Antibody [NBP1-50033] - Detection of Human IBtk by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-IBtk antibody used for WB at 0.1 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. IBtk was also immunoprecipitated by rabbit anti-IBtk antibody BL10880, which recognizes an upstream epitope. For blotting immunoprecipitated IBtk, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).