![Western Blot: ANKRD27 Antibody [NBP1-50030] - Detection of Human Varp by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Varp antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Varp was also immunoprecipitated by rabbit anti-Varp antibody which recognizes an upstream epitope. For blotting immunoprecipitated Varp, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_9/29765_ANKRD27-Antibody-Western-Blot-NBP1-50030-img0003.jpg)
Western Blot: ANKRD27 Antibody [NBP1-50030] - Detection of Human Varp by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Varp antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Varp was also immunoprecipitated by rabbit anti-Varp antibody which recognizes an upstream epitope. For blotting immunoprecipitated Varp, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).