ANKRD27

Gene Symbol ANKRD27
Entrez Gene 84079
Alt Symbol PP12899, VARP
Species Human
Gene Type protein-coding
Description ankyrin repeat domain 27 (VPS9 domain)
Other Description VPS9 domain-containing protein|VPS9-ankyrin-repeat protein|Vps9 domain and ankyrin-repeat-containing protein|ankyrin repeat domain-containing protein 27
Swissprots Q96NW4 Q9H0I4 Q86UC3 Q8ND80 Q71MF5
Accessions Q96NW4 AA903176 AF447882 AAQ04657 AK054561 BAB70755 AL136784 CAB66718 AL834335 CAD39003 AM393863 CAL38738 BC015552 BC045605 BC050529 AAH50529 BI462844 XM_005259310 XP_005259367 XM_006723412 XP_006723475 NM_032139 NP_115515
Function May be a guanine exchange factor (GEF) for Rab21, Rab32 and Rab38 and regulate endosome dynamics (PubMed:16525121, PubMed:18477474). May regulate the participation of VAMP7 in membrane fusion events; in vitro inhibits VAMP7-mediated SNARE complex formation by trapping VAMP7 in a closed, fusogenically inactive conformation (PubMed:23104059). Involved in peripheral melanosomal distribution of TYRP1 in melanocytes; the function, which probably is implicating vesicle-trafficking, includes cooperation with Rab32, Rab38 and VAMP7 (By similarity). Involved in the regulation of neurite growth; the function seems to require its GEF activity, probably towards Rab21, and VAMP7 but not Rab32/38 (By similarity). Proposed to be involved in Golgi sorting of VAMP7 and transport of VAMP7 vesicles to the cell surface; the function seems to implicate kinesin heavy chain isoform 5 proteins, GOLGA4, RAB21 and MACF1 (PubMed:22705394). Required for the colocalization of VAMP7 and Rab21, probably on TGN sites
Subcellular Location Early endosome {ECO:0000269|PubMed:16525121, ECO:0000269|PubMed:24856514}. Late endosome {ECO:0000269|PubMed:23104059}. Cytoplasmic vesicle membrane {ECO:0000269|PubMed:23104059}. Lysosome {ECO:0000269|PubMed:23104059}. Cell membrane {ECO:0000269|PubMed:23104059}. Melanosome {ECO:0000250|UniProtKB:Q3UMR0}. Note=Colocalizes with VAMP7 in transport vesicles in the shaft of hippoocampal neurons (By similarity). {ECO:0000250|UniProtKB:Q3UMR0}.

Anti-VARP antibody - ab108216 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab108216 stained HeLa cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab108216 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-VARP antibody - ab124203 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-VARP antibody (ab124203) at 1 µg/mlLane 2 : Anti-VARP antibody (ab124203) at 2 µg/mlLane 1 : K562 cell lysate Lane 2 : K562 cell lysate Lysates/proteins at 15 µg per lane.

Anti-VARP antibody - ab113832 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab113832, at 5µg/ml, staining VARP in Formalin-fixed, Paraffin-embedded Human Small Intestine tissue by Immunohistochemistry.

Anti-ANKRD27 Antibody (aa225-275) - LS-C289704 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ANKRD27 Antibody (aa1000-1050) - LS-C289705 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ANKRD27 Antibody (aa640-689) - LS-C135499 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ANKRD27 antibody LS-C135499 Western blot of MCF7 Cell lysate. Antibody concentration 1 ug/ml.

Anti-ANKRD27 Antibody (N-Terminus) - LS-C144478 from LifeSpan Bioscience

Prices $325.00, $890.00
Sizes 100 µg, 500 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of VARP in K562 cell lysate with VARP antibody at (A) 1 and (B) 2 ug/ml.

Anti-ANKRD27 Antibody (Internal) IHC-plus™ - LS-B4720 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-ANKRD27 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4720 concentration 5 ug/ml.

ANKRD27 Antibody - NBP1-50030 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: ANKRD27 Antibody [NBP1-50030] - Detection of Human Varp by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Varp antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Varp was also immunoprecipitated by rabbit anti-Varp antibody which recognizes an upstream epitope. For blotting immunoprecipitated Varp, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

ANKRD27 Antibody - NBP1-50029 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunoprecipitation: ANKRD27 Antibody [NBP1-50029] - Detection of Human Varp by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Varp antibody used for IP at 6 mcg/mg lysate. Varp was also immunoprecipitated by rabbit anti-Varp antibody which recognizes a downstream epitope. For blotting immunoprecipitated Varp was used at 1 mcg/ml, and the ReliaBLOT Reagents and Procedures were incorporated. Detection: Chemiluminescence with an exposure time of 30 seconds.

ANKRD27 Antibody - NBP1-85111 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry-Paraffin: ANKRD27 Antibody [NBP1-85111] - Staining of human thyroid gland shows cytoplasmic positivity in glandular cells.

Anti-ANKRD27 antibody produced in rabbit - SAB2900823 from Sigma-Aldrich

Prices $465.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-ANKRD27 (C-Terminus), antibody produced in rabbit: <B>Cat. No. SAB2900823</B>: Anti-ANKRD27 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 μg/mL.

Anti-ANKRD27 antibody produced in rabbit - SAB3500700 from Sigma-Aldrich

Prices $341.00
Sizes 100 µg
Host Rabbit
<B>Immunohistochemistry</B><BR/>Anti-ANKRD27; <B>Cat. No. SAB3500700</B>; Immunohistochemistry of VARP in Human small intestine with VARP antibody at 5 μ/mL.

Anti-ANKRD27 antibody produced in rabbit - SAB2107522 from Sigma-Aldrich

Prices $341.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-ANKRD27: <B>Cat. No. SAB2107522</B>: Western blot analysis of ANKRD27 in MCF7 with ANKRD27 (ankyrin repeat domain 27, VPS9 domain) antibody, against the C terminal of ANKRD27 (50 μg) antibody at 1.0 μg/mL.

Anti-ANKRD27 antibody produced in rabbit - SAB3500698 from Sigma-Aldrich

Prices $341.00
Sizes 100 µg
Host Rabbit
<B>Immunoblotting</B><BR/>Anti-ANKRD27; <B>Cat. No. SAB3500698</B>; Western blot analysis of VARP in K562 cell lysate with VARP antibody at (A) 1 and (B) 2 μ/mL.

Anti-ANKRD27 antibody produced in rabbit - HPA042769 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-ANKRD27: <B>Cat. No. HPA042769</B>: Immunohistochemical staining of human colon shows strong membranous and cytoplasmic positivity in glandular cells.

ANKRD27 antibody - C-terminal region (ARP60395_P050) - ARP60395_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

ANKRD27 antibody, C-term - GTX48931 from GeneTex

Prices $329.00
Sizes 50 μg
Host Rabbit
Clonality Polyclonal
Anti-ANKRD27 antibody, C-term used in Western blot. GTX48931

VARP antibody - orb75723 from Biorbyt

Prices $723.00
Sizes 100 μg
Host Rabbit
Clonality Polyclonal
Western blot analysis of K562 cell lysate at (A) 1 and (B) 2 ug/mL using VARP antibody

VARP antibody - orb75296 from Biorbyt

Prices $723.00
Sizes 100 μg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed and paraffin-embedded human small intestine at 5 ug/mL using VARP antibody

ANKRD27 antibody - 70R-10062 from Fitzgerald

Prices $345.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

ANKRD27 antibody - AP23548PU-N from Acris Antibodies

Prices $430.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

ANKRD27 (Center) antibody - AP55658PU-N from Acris Antibodies

Prices $340.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of VARP in human small intestine with VARP antibody Cat.-No AP55658PU-N at 5 µg/ml.

ANKRD27 (N-term) antibody - AP55668PU-N from Acris Antibodies

Prices $340.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of VARP in K562 cell lysate with VARP antibody Cat.-No AP55668PU-N at (A) 1 and (B) 2 μg/mL.
Search more