Immunoprecipitation: ANKRD27 Antibody [NBP1-50029] - Detection of Human Varp by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Varp antibody used for IP at 6 mcg/mg lysate. Varp was also immunoprecipitated by rabbit anti-Varp antibody which recognizes a downstream epitope. For blotting immunoprecipitated Varp was used at 1 mcg/ml, and the ReliaBLOT Reagents and Procedures were incorporated. Detection: Chemiluminescence with an exposure time of 30 seconds.