Western Blot: PARC Antibody [NB100-2300] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PARC/H7-AP1 antibody (lot was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: PARC Antibody [NB100-2300] - Detection of Human PARC/H7-AP1 on HeLa whole cell lysate using NB100-2300. Normal rabbit IgG was used as a control for immunoprecipitation (IP Control).
Western Blot: PARC Antibody [NB100-2300] - Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PARC/H7-AP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.