Western blot analysis of extracts from various cell lines using UBE2T (D2L7H) Rabbit mAb.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human UBE2T (hUBE2T-Myc/DDK; +), using UBE2T (D2L7H) Rabbit mAb.
Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-) or SignalSilence ® UBE2T siRNA I #13142 (+), using UBE2T (D2L7H) Rabbit mAb (upper) or GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower). The UBE2T (D2L7H) Rabbit mAb confirms silencing of UBE2T expression, while the GAPDH (D16H11) XP ® Rabbit mAb is used as a loading control.
Immunoprecipitation of UBE2T from 293T cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or UBE2T (D2L7H) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using UBE2T (D2L7H) Rabbit mAb.