![Western Blot: NIPBL Antibody [NBP1-77974] - Detection of a band ~300 kDa corresponding to IDN3 (arrowhead) in human kidney (lane 1) and human heart (lane 2) whole cell tissue extracts. Approximately 20ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:500. Use HRP conjugated Rb-a-Goat IgG [H&L] MXHu for detection.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_10/5078_NIPBL%2520Antibody-Western%2520Blot-NBP1-77974-img0002.jpg)
Western Blot: NIPBL Antibody [NBP1-77974] - Detection of a band ~300 kDa corresponding to IDN3 (arrowhead) in human kidney (lane 1) and human heart (lane 2) whole cell tissue extracts. Approximately 20ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:500. Use HRP conjugated Rb-a-Goat IgG [H&L] MXHu for detection.
![Western Blot: NIPBL Antibody [NBP1-77974] - Shows detection of bands at ~315 kDa and ~125 kDa corresponding to isoforms of IDN3 (arrow-heads) in mouse heart whole cell tissue extract. Approximately 35 ug of lysate was separated on a 4-8% gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500. Reaction occurred overnight at 4h followed by washes and reaction with a 1:20,000 dilution of IRDye (TM) 800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_10/5079_NIPBL-Antibody-Western-Blot-NBP1-77974-img0001.jpg)
Western Blot: NIPBL Antibody [NBP1-77974] - Shows detection of bands at ~315 kDa and ~125 kDa corresponding to isoforms of IDN3 (arrow-heads) in mouse heart whole cell tissue extract. Approximately 35 ug of lysate was separated on a 4-8% gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500. Reaction occurred overnight at 4h followed by washes and reaction with a 1:20,000 dilution of IRDye (TM) 800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature.