NIPBL

Gene Symbol NIPBL
Entrez Gene 25836
Alt Symbol CDLS, CDLS1, IDN3, IDN3-B, Scc2
Species Human
Gene Type protein-coding
Description Nipped-B homolog (Drosophila)
Other Description SCC2 homolog|delangin|nipped-B-like protein|sister chromatid cohesion 2 homolog
Swissprots Q6N080 Q6KCD6 Q6ZT92 Q9Y6Y4 Q9Y6Y3 Q8N4M5 Q6KC79 Q7Z2E6
Accessions EAW55948 EAW55949 EAW55950 Q6KC79 AA508213 AJ627032 CAF25290 AJ640137 CAG26691 AK002065 AK022659 AK023416 AK023710 AK126804 BAC86701 AK290347 BAF83036 AL137726 AY726600 BC032711 BC033847 AAH33847 BC063859 BC131490 AAI31491 BC146821 AAI46822 BK005151 DAA05331 BQ723324 BX538177 CAD98051 BX538178 CAD98052 BX640644 CAE45790 CN369580 KJ807789 KJ807790 KJ807791 KJ807792 XM_005248280 XP_005248337 XM_005248282 XP_005248339 XM_006714467 XP_006714530 XM_006714468 XP_006714531 XM_011514014 XP_011512316 XM_011514015 XP_011512317 NM_015384 NP_056199 NM_133433 NP_597677
Function Probably plays a structural role in chromatin. Involved in sister chromatid cohesion, possibly by interacting with the cohesin complex (By similarity). {ECO:0000250}.
Subcellular Location Nucleus {ECO:0000250}.
Tissue Specificity Widely expressed. Highly expressed in heart, skeletal muscle, fetal and adult liver, fetal and adult kidney. Expressed at intermediates level in thymus, placenta, peripheral leukocyte and small intestine. Weakly or not expressed in brain, colon, spleen and lung. {ECO:0000269|PubMed:15146185, ECO:0000269|PubMed:15146186}.

NIPBL (T-20) - sc-69440 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

NIPBL (C-9) - sc-374625 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NIPBL (C-9): sc-374625. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

NIPBL (S-20) - sc-69439 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NIPBL (S-20): sc-69439. Western blot analysis of NIPBL expression in HeLa (A) and HEK293 (B) whole cell lysates.

NIPBL (N-20) - sc-69438 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

NIPBL (I-17) - sc-69437 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

NIPBL (H-300) - sc-98601 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-IDN3 antibody - ab57054 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab57054 IDN3 antibody at 1ug/ml.

Anti-IDN3 antibody [KT55] - ab106768 from Abcam

Prices $359.00
Sizes 100 µl
Host Rat
Clonality Monoclonal
Clone KT55
Overlay histogram showing HepG2 cells stained with ab106768 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab106768, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rat IgG (H+L) (ab150165) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-IDN3 / NIPBL Antibody (aa889-902) - LS-C112999 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-IDN3 / NIPBL Antibody (aa344-356) - LS-C60065 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-IDN3 Antibody - Western Blot. Western blot of Affinity Purified anti-IDN3 antibody shows detection of bands at ~315 kD and ~125 kD corresponding to isoforms of IDN3 (arrow-heads) in mouse heart whole cell tissue extract. Approximately 35 ug of lysate was separated on a 4-8% gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1500. Reaction occurred overnight at 4? followed by washes and reaction with a 1:20000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MXHu ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Anti-IDN3 / NIPBL Antibody (aa1025-1075) - LS-C126032 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-IDN3 / NIPBL Antibody (clone KT55) - LS-C193504 from LifeSpan Bioscience

Host Rat
Clonality Monoclonal
Clone KT55

Anti-IDN3 / NIPBL Antibody (aa550-600) - LS-C288559 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-IDN3 / NIPBL Antibody (clone KT54) - LS-C193503 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Rat
Clonality Monoclonal
Clone KT54

Anti-IDN3 / NIPBL Antibody IHC-plus™ - LS-B7393 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rat
Clonality Monoclonal
Anti-NIPBL antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7393 concentration 10 ug/ml.

Anti-IDN3 / NIPBL Antibody (clone KT54) IHC-plus™ - LS-B7392 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rat
Clonality Monoclonal
Clone KT54
Anti-NIPBL antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7392 concentration 10 ug/ml.

Anti-IDN3 / NIPBL Antibody (aa1025-1075) - LS-C288560 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human NIPBL by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NIPBL antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. NIPBL was also immunoprecipitated by a prior lot of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

NIPBL Antibody - NBP1-85110 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

NIPBL Antibody - NBP1-77974 from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot: NIPBL Antibody [NBP1-77974] - Detection of a band ~300 kDa corresponding to IDN3 (arrowhead) in human kidney (lane 1) and human heart (lane 2) whole cell tissue extracts. Approximately 20ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:500. Use HRP conjugated Rb-a-Goat IgG [H&L] MXHu for detection.

NIPBL Antibody - NBP1-52168 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

NIPBL Antibody (3B9) - H00025836-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3B9
Immunocytochemistry/Immunofluorescence: NIPBL Antibody (3B9) [H00025836-M01] - Analysis of monoclonal antibody to NIPBL on HeLa cell. Antibody concentration 10 ug/ml.

NIPBL Antibody - NB100-93320 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: NIPBL Antibody [NB100-93320] - Detection of Human NIPBL by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-NIPBL antibody NB100-93320 used for IP at 3 mcg/mg lysate. NIPBL was also immunoprecipitated by rabbit anti- NIPBL antibody NB100-93319, which recognizes an upstream epitope

IDN3 Polyclonal Antibody - bs-15541R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NIPBL antibody produced in rabbit - HPA040834 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-NIPBL: <B>Cat. No. HPA040834</B>: Immunohistochemical staining of human thyroid gland shows strong nuclear positivity in glandular cells.

Monoclonal Anti-NIPBL antibody produced in mouse - WH0025836M1 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3B9
<B>Immunoblotting</B><BR/>Monoclonal Anti-NIPBL, <B>Cat. No. WH0025836M1</B> used at the antibody concentration: 1 μg/mL. Specific band of ~35.1 kDa using immunogen protein lysate.
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