Western blot analysis of extracts from U-937 cells, untreated (-) or treated with TPA #4174 (200 nM, 72 hr; +), using uPAR (D4Q5S) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunoprecipitation of uPAR from U-937 cell extracts treated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (200 nM, 72 hr; +) using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or uPAR (D4Q5S) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using uPAR (D4Q5S) Rabbit mAb as the primary antibody and Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 as the secondary antibody.