USP9X (D4Y7W) Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 14898
• Prices: $246.00
• Sizes: 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D4Y7W
• Applications: WB IP IHC-P ICC/IF
• Species Reactivities: Human, Mouse, Rat, Monkey, Bovine, Dog, Horse
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn1272 of human USP9X protein.
• Description: Rabbit Monoclonal
• Gene: USP9X

Product images

Western blot analysis of extracts from various cell lines using USP9X (D4Y7W) Rabbit mAb (upper) and GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower). LOX-IMVI cells are deficient in USP9X protein expression (13).

Immunoprecipitation of USP9X from 293T cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or USP9X (D4Y7W) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using USP9X (D4Y7W) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded PC-3 (left, positive) and LOX-IMVI (right, negative) cell pellets using USP9X (D4Y7W) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using USP9X (D4Y7W) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using USP9X (D4Y7W) Rabbit mAb.

Confocal immunofluorescent analysis of 293T (left, positive) and LOX-IMVI (right, negative) cells using USP9X (D4Y7W) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).