Western Blot: BRCC3 Antibody (3B1) [NBP1-30429] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BRCC36 (1:1000). Proteins were visualized using a goat ant imouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: HepG2 cell lysate Lane 3.: 293T cell lysate Lane 4.: MCF7 cell lysate Lane 5.: NIH3T3 cell lysate.
Immunocytochemistry/Immunofluorescence: BRCC3 Antibody (3B1) [NBP1-30429] - CC/IF analysis of BRCC36 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BRCC36 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Flow Cytometry: BRCC3 Antibody (3B1) [NBP1-30429] - Flow cytometry analysis of BRCC36 in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).