Western Blot: CISD1 Antibody (1A8) [NBP2-22567] - The cell lysates (40ug) were resolved by SDS-PAGE,transferred to PVDF membrane and probed with anti-humanCISD1 antibody (1:1000). Proteins were visualized using agoat anti-mouse secondary antibody conjugated to HRP andan ECL detection system.Lane 1.: K562 cell lysateLane 2.: MCF7 cell lysat
Flow Cytometry: CISD1 Antibody (1A8) [NBP2-22567] - Analysis of CISD1 in THP-1 cell line, stainingat 2-5ug for 1x106cells (red line). The secondary antibodyused goat anti-mouse IgG Alexa fluor 488 conjugate. Isotypecontrol antibody was mouse IgG (black line).
Western Blot: CISD1 Antibody (1A8) [NBP2-22567] - The extract of Mouse liver (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CISD1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody.