CISD1

Gene Symbol	CISD1
Entrez Gene	55847
Alt Symbol	C10orf70, MDS029, ZCD1, mitoNEET
Species	Human
Gene Type	protein-coding
Description	CDGSH iron sulfur domain 1
Other Description	CDGSH iron sulfur domain-containing protein 1\|CDGSH iron-sulfur domain-containing protein 1\|zinc finger CDGSH-type domain 1\|zinc finger, CDGSH-type domain 1
Swissprots	Q9NZ45 Q1X902
Accessions	EAW54162 EAW54163 EAW54164 EAW54165 EAW54166 Q9NZ45 AF220049 AAF67642 AI025360 AK312017 BAG34955 AY960578 AAY32336 BC005962 AAH05962 BC007043 AAH07043 BC008474 AAH08474 BC059168 AAH59168 DA417986 HQ448690 ADQ33168 NM_018464 NP_060934
Function	Plays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation (By similarity). May be involved in Fe-S cluster shuttling and/or in redox reactions. {ECO:0000250, ECO:0000269\|PubMed:17584744, ECO:0000269\|PubMed:17766440}.
Subcellular Location	Mitochondrion outer membrane {ECO:0000269\|PubMed:17376863, ECO:0000269\|PubMed:17766439, ECO:0000269\|PubMed:17905743, ECO:0000269\|PubMed:18047834}; Single- pass type III membrane protein {ECO:0000269\|PubMed:17376863, ECO:0000269\|PubMed:17766439, ECO:0000269\|PubMed:17905743, ECO:0000269\|PubMed:18047834}.
Tissue Specificity	Expression is reduced in cells derived from cystic fibrosis patients. {ECO:0000269\|PubMed:18047834}.

mitoNEET (D-13) - sc-138987 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

Anti-CISD1 antibody - ab203096 from Abcam

Prices	$359.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat stomach tissue labeling CISD1 with ab203096 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Anti-CISD1 antibody [2B3] - ab118027 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	2B3

All lanes : Anti-CISD1 antibody [2B3] (ab118027) at 1/500 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY CISD1 cDNALysates/proteins at 5 µg per lane.

Anti-CISD1 Antibody (clone AT1A8) IHC-plus™ - LS-B9661 from LifeSpan Bioscience

Prices	$395.00
Sizes	50 µl
Host	Mouse
Clonality	Monoclonal
Clone	AT1A8

Anti-CISD1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9661 concentration 10 ug/ml.

Anti-CISD1 Antibody (clone 3A1) - LS-C114965 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	3A1

IHC of paraffin-embedded Carcinoma of prostate tissue using anti-CISD1 mouse monoclonal antibody. (Dilution 1:50).

Anti-CISD1 Antibody (C-Terminus) - LS-C152029 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

CISD1 Antibody (4G3) - NBP2-02321 from Novus Biologicals

Prices	$329.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	4G3

Western Blot: CISD1 Antibody (4G3) [NBP2-02321] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CISD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CISD1.

CISD1 Antibody (1A8) - NBP2-22567 from Novus Biologicals

Prices	$279.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	1A8

Western Blot: CISD1 Antibody (1A8) [NBP2-22567] - The cell lysates (40ug) were resolved by SDS-PAGE,transferred to PVDF membrane and probed with anti-humanCISD1 antibody (1:1000). Proteins were visualized using agoat anti-mouse secondary antibody conjugated to HRP andan ECL detection system.Lane 1.: K562 cell lysateLane 2.: MCF7 cell lysat

CISD1 Polyclonal Antibody - bs-13959R from Bioss

Prices	$269.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Formalin-fixed and paraffin embedded rat stomach labeled with Rabbit Rabbit Anti-CISD1 Polyclonal Antibody, Unconjugated (bs-13959R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Anti-CISD1 (84-97) antibody produced in rabbit - SAB1102366 from Sigma-Aldrich

Prices	$298.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human CISD1 was separated on SDS-PAGE, blotted with Anti-CISD1 (84-97) (<B>Cat. No. SAB1102366</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:250

Monoclonal Anti-CISD1 antibody produced in mouse - SAB4100154 from Sigma-Aldrich

Prices	$358.00
Sizes	200 µl
Host	Mouse
Clonality	Monoclonal
Clone	CISD1-A122

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells expressing FLAG<SUP>®</SUP>-tagged human CISD1 was separated on SDS-PAGE, probed with Mouse Anti-CISD1 Clone: CISD1-A122 (<B>Cat. No. SAB4100154</B>). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (<B>Cat. No. A2304</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:1,000<BR/>2. Antibody dilution 1:2,000<BR/>3. Negative control<BR/>4. Positive control Anti-FLAG<SUP>®</SUP>

CISD1 Antibody - C-terminal region (ARP65647_P050) - ARP65647_P050 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

CISD1 antibody [2B3] - GTX84683 from GeneTex

Prices	$329.00
Sizes	100 μl
Host	Mouse
Clonality	Monoclonal
Clone	2B3

Anti-CISD1 antibody [2B3] used in Flow cytometry/FACS. GTX84683

CISD1 antibody [3A1] - GTX84682 from GeneTex

Prices	$369.00
Sizes	100 μl
Host	Mouse
Clonality	Monoclonal
Clone	3A1

Anti-CISD1 antibody [3A1] used in IHC (Formalin-fixed paraffin-embedded sections). GTX84682

CISD1 antibody [4G3] - GTX84684 from GeneTex

Prices	$329.00
Sizes	100 μl
Host	Mouse
Clonality	Monoclonal
Clone	4G3

Anti-CISD1 antibody [4G3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX84684

CISD1 Antibody (OABF00677) - OABF00677 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

CISD1 antibody - 10R-8663 from Fitzgerald

Prices	$175.00
Sizes	50 µl
Host	Mouse
Clonality	Monoclonal
Clone	AT1A8

CISD1 antibody - 10R-3691 from Fitzgerald

Prices	$395.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	3A1

CISD1 antibody - 10R-3690 from Fitzgerald

Prices	$495.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	2B3

Anti -CISD1 (CDGSH Iron-Sulfur Domain-Containing Protein 1) - 218038 from USBiological Life Science

Host	Mouse
Clonality	Monoclonal
Clone	15B11

CISD1 antibody - 70R-21600 from Fitzgerald

Prices	$375.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

Cdgsh Iron Sulfur Domain 1 Antibody - abx111509 from Abbexa

Prices	$490.00, $490.00, $490.00, $490.00
Sizes	10 μl, 50 μl, 100 μl, 200 μl
Host	Rabbit
Clonality	Polyclonal

CISD1 antibody - 16006-1-AP from Acris Antibodies

Prices	$270.00
Sizes	150 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemical of paraffin-embedded human heart using 16006-1-AP(mitoNEET,CISD1 antibody) at dilution of 1:100 (under 10x lens)

CISD1 antibody - AM50028PU-N from Acris Antibodies

Prices	$350.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	AT1A8

Western Blot: The extract of Mouse liver (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CISD1 antibody (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody.

CISD1 antibody - AM50028PU-S from Acris Antibodies

Prices	$255.00
Sizes	50 µl
Host	Mouse
Clonality	Monoclonal
Clone	AT1A8

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