Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human VCAM-1 (hVCAM-1-Myc/DDK; +), using VCAM-1 (E1E8X) Rabbit mAb.
Western blot analysis of extracts from HUVEC cells, untreated (-) or treated with Human Tumor Necrosis Factor-α (hTNF-α) #8902 (10 ng/ml, 16 hr; +), using VCAM-1 (E1E8X) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Flow cytometric analysis of fixed, non-permeabilized HUVEC cells, untreated (blue) or treated with Human Tumor Necrosis Factor-α (hTNF-α) #8902 (10 ng/ml, 16 hr; green) using VCAM1 (E1E8X) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary antibody.