Western blot analysis of extracts from HUVEC cells, untreated (-) or treated with Human Tumor Necrosis Factor-α (hTNF-α) #8902 (10 ng/ml, 16 hr; +), using VCAM-1 Antibody (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human VCAM-1 (hVCAM-1-Myc/DDK; +), using VCAM-1 Antibody.
Immunoprecipitation of VCAM-1 from HUVEC extracts, treated with Human Tumor Necrosis Factor-α (hTNF-α) #8902 (10 ng/ml, 16 hr), using Normal Rabbit IgG #2729 (lane 2) or VCAM-1 Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using VCAM-1 Antibody.