Western Blot: Nitrilase 1 Antibody (1C3) [H00004817-M01] - Analysis of NIT1 expression in transfected 293T cell line by NIT1 monoclonal antibody (M01), clone 1C3.Lane 1: NIT1 transfected lysate (Predicted MW: 35.9 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: Nitrilase 1 Antibody (1C3) [H00004817-M01] - Analysis of monoclonal antibody to NIT1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunoprecipitation: Nitrilase 1 Antibody (1C3) [H00004817-M01] - Analysis of NIT1 transfected lysate using anti-NIT1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NIT1 monoclonal antibody.
Sandwich ELISA: Nitrilase 1 Antibody (1C3) [H00004817-M01] - Detection limit for recombinant GST tagged NIT1 is 1 ng/ml as a capture antibody.