Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Geldanamycin #9843 (+), using Wee1 (D10D2) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from various cell lines using Wee1 (D10D2) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Wee1 (D10D2) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded HT-29 (left) or PC-3 (right) cell pellets using Wee1 (D10D2) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Wee1 (D10D2) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Flow cytometric analysis of Jurkat cells using Wee1 (D10D2) Rabbit mAb and Propidium Iodide (PI)/RNase Staining Solution #4087 to measure DNA content. Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary antibody.