WEE1

Gene Symbol WEE1
Entrez Gene 7465
Alt Symbol WEE1A, WEE1hu
Species Human
Gene Type protein-coding
Description WEE1 G2 checkpoint kinase
Other Description WEE1 homolog|WEE1+ homolog|wee1-like protein kinase|wee1A kinase
Swissprots B3KVE1 P30291 D3DQV0
Accessions CAC14173 EAW68586 EAW68587 EAW68588 P30291 AK122837 BAG53753 BC051831 AAH51831 BC070052 AAH70052 BQ024511 BX641032 CAE46020 EU832325 ACE87582 EU832410 ACE86895 U10564 AAB60401 X62048 CAA43979 XM_005253118 XP_005253175 XM_005253119 XP_005253176 NM_001143976 NP_001137448 NM_003390 NP_003381
Function Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Subcellular Location Nucleus.
Top Pathways Cell cycle

Phospho-Wee1 (Ser642) (D47G5) Rabbit mAb - 4910 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D47G5
Western blot analysis of extracts from A431 and H441 cells, untreated or EGF-treated, using Phospho-Wee1 (Ser642) (D47G5) Rabbit mAb (upper) or Wee1 Antibody #4936 (lower).

Wee1 (D10D2) Rabbit mAb - 13084 from Cell Signaling Technology

Prices $99.00, $250.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D10D2
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Geldanamycin #9843 (+), using Wee1 (D10D2) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Wee1 Antibody - 4936 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa cells, untreated or Lambda Phosphatase-treated NEB #P0753 (10,000 units/ml for 1h), using Wee1 Antibody.

Wee 1 (B-11) - sc-5285 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Wee 1 CRISPR/Cas9 KO Plasmid (h): sc-400701. Western blot analysis of human Wee 1 expression in empty vector control (A) and Wee 1 CRISPR/Cas9 KO Plasmid transfected (B) HEK293T whole cell lysates. Blot probed with Wee 1 (B-11): sc-5285. α-actinin (H-2): sc-17829 used as specificity and loading control.

Wee 1 (C-20) - sc-325 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Wee 1 (C-20): sc-325. Western blot analysis of Wee 1 in Jurkat (A) and K-562 (B) nuclear extracts and BJAB (C) whole cell lysate.

Wee 1 (H-300) - sc-9037 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of Wee 1 expression in MOLT-4 (A), BJAB (B,D) and WEHI-231 (C) whole cell lysates. Antibodies tested include Wee 1 (B-11): sc-5285 (A-C) and Wee 1 (H-300): sc-9037 (D).

Anti-Wee1 (phospho S123) antibody - ab60034 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ab60034 at a dilution of 1/50. A peroxidase-conjugated secondary antibody was used, followed by AEC staining.

Anti-Wee1 (phospho S53) antibody - ab135557 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-Wee1 (phospho S53) antibody (ab135557) at 1/100 dilution + Mouse liver tissue lysate at 12.5 µg

Anti-Wee1 antibody - ab2315 from Abcam

Prices $372.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-Wee1 antibody - ab203236 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Wee1 antibody (ab203236) at 1/200 dilutionLane 1 : Rat heart lysateLane 2 : Rat brain lysateSecondaryGoat Anti-Rabbit IgG Antibody (H+L), HRP at 1/3000 dilution

Anti-Wee1 antibody - ab79298 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Wee1 antibody (ab79298) at 1/500 dilutionLane 1 : Extracts from 293 cellsLane 2 : Extracts from 293 cells with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-Wee1 antibody - ab58059 from Abcam

Host Mouse
Clonality Monoclonal

Anti-Wee1 antibody - Carboxyterminal end - ab37597 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Wee1 antibody - Carboxyterminal end (ab37597) + HL-60 cell lysateSecondaryHRP-anti-Rabbitdeveloped using the ECL technique

Anti-WEE1 Antibody (C-Terminus) - LS-C11059 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-WEE1 Antibody (phospho-Ser53) - LS-C199732 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded human breast carcinoma, using WEE1 (Phospho-Ser53) Antibody. The sample on the right was incubated with synthetic peptide.

Anti-WEE1 Antibody (aa19-68) - LS-C118328 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from 293 cells, using WEE1 (Ab-53) Antibody. The lane on the right is treated with the synthesized peptide.

Anti-WEE1 Antibody (clone 5B6) - LS-C198441 from LifeSpan Bioscience

Prices $355.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal
Clone 5B6
WEE1 monoclonal antibody (M01A), clone 5B6 Western Blot analysis of WEE1 expression in HeLa NE.

Anti-WEE1 Antibody (C-Terminus) - LS-C204262 from LifeSpan Bioscience

Prices $355.00
Sizes 600 µg
Host Goat
Clonality Polyclonal

Anti-WEE1 Antibody (aa202-230, AP) - LS-C273571 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-WEE1 Antibody (aa202-230, APC) - LS-C273572 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-WEE1 Antibody (aa202-230, Biotin) - LS-C273573 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-WEE1 Antibody (aa202-230, FITC) - LS-C273574 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-WEE1 Antibody (aa202-230, Azide-free, HRP) - LS-C273575 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-WEE1 Antibody (aa202-230) - LS-C158286 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Mouse Wee1 Antibody western blot of WiDr cell line lysates (35 ug/lane). The Wee1 antibody detected the Wee1 protein (arrow).

Anti-WEE1 Antibody - LS-C136965 from LifeSpan Bioscience

Prices $255.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Search more