Western blot analysis of extracts from Raji and KARPAS-299 cells, untreated (-) or treated with TPA #4174 (200 nM, 30 min; +) using Phospho-WIPI2 (Ser413) Antibody (upper) or total WIPI2 Antibody #8567 (lower).
Western blot analysis of extracts from KARPAS-299 cells, treated with TPA #4174 (200 nM, 30 min) and then untreated (-) or λ phosphatase and calf intestinal phosphatase (CIP)-treated (+), using Phospho-WIPI2 (Ser413) Antibody (upper) or total WIPI2 Antibody #8567 (lower).
Immunoprecipitation of phospho-WIPI2 (Ser413) from KARPAS-299 cells treated with TPA #4174 (200 nM, 30 min) using Normal Rabbit IgG #2729 (lane 2) or Phospho-WIPI2 (Ser413) Antibody (lane 3). Lane 1 represents 10% input. Western blot was perform using Phospho-WIPI2 (Ser413) Antibody. Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 was used as a secondary antibody.