Western Blot: KNTC1 Antibody [NB100-88131] - Detection of Human ROD/KNTC1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ROD/KNTC1 antibody NB100-88131 used for IP at 3 mcg/mg lysate. ROD/KNTC1 was also immunoprecipitated by rabbit anti-ROD/KNTC1 antibody NB100-88130, which recognizes an upstream epitope. Detection: Chemiluminescence with an exposure time of 10 seconds. (Lower bands that were immunoprecipitated by both NB100-88130 and NB100-88131 are marked by asterisks.)