Immunohistochemistry: O-GlcNAcase/OGA/MGEA5 Antibody [NBP2-32233] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti- MGEA5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Immunoprecipitation: O-GlcNAcase/OGA/MGEA5 Antibody [NBP2-32233] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MGEA5 antibody NBP2-32233 used for IP at 6 ug per reaction. MGEA5 was also immunoprecipitated by rabbit anti-MGEA5 antibody BL15772. For blotting immunoprecipitated MGEA5, NBP2-32233 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.