Western Blot: PCNA associated factor Antibody [NBP2-32117] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-PAF antibody NBP2-32117 used for IP at 6 ug per reaction. PAF was also immunoprecipitated by rabbit anti-PAF antibody NBP2-32117. For blotting immunoprecipitated PAF, NBP2-32117 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry: PCNA associated factor Antibody [NBP2-32117] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PAF used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).