Phospho-Sin1 (Thr86) (D4U9L) Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 14716
• Prices: $287.00
• Sizes: 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D4U9L
• Applications: WB IP
• Species Reactivities: Human, Mouse, Rat
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding phosphorylated Thr86 of human Sin1 protein.
• Description: Rabbit Monoclonal
• Gene: MAPKAP1

Product images

Western blot analysis of extracts from serum starved NIH/3T3 cells, untreated (-) or treated with insulin (150 nM, 5 min; +) or Human Insulin-like Growth Factor I (hIGF-I) #8917 (100 ng/ml, 5 min; +) in the absence (-) or presence (+) of λ phosphatase, using Phospho-Sin1 (Thr86) (D4U9L) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western blot analysis of extracts from serum starved HeLa cells, untreated (-) or treated with Human Insulin-like Growth Factor I (hIGF-I) #8917 (100 ng/ml, 5 min; +) in the absence (-) or presence (+) of λ phosphatase, using Phospho-Sin1 (Thr86) (D4U9L) Rabbit mAb (upper) and Sin1 (D7G1A) Rabbit mAb #12860 (lower).

Immunoprecipitation of phospho-Sin1 (Thr86) from serum starved NIH/3T3 cell extracts treated with insulin (150 nM, 5 min) using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or Phospho-Sin1 (Thr86) (D4U9L) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-Sin1 (Thr86) (D4U9L) Rabbit mAb.