Fas Ligand/TNFSF6 Antibody (H11) [Biotin]
| Name | Fas Ligand/TNFSF6 Antibody (H11) [Biotin] |
|---|---|
| Supplier | Novus Biologicals |
| Catalog | NBP1-97621 |
| Prices | $269.00 |
| Sizes | 50 tests |
| Host | Rat |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | H11 |
| Applications | WB FC ICC/IF |
| Species Reactivities | Mouse |
| Antigen | A synthetic peptide corresponding to amino acids 196-220 of mouse Fasl. |
| Purity/Format | Affinity purified |
| Description | Rat Monoclonal |
| Gene | Fasl |
| Conjugate | Biotin (A=N/A, E=N/A) |
| Supplier Page | Shop |
Product images
![Immunocytochemistry/Immunofluorescence: Fas Ligand Antibody (H11) [Biotin] [NBP1-97621] - Detection of FasL in 293T cells transfected with a FasL expression vector. Method: Transfected cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20C for 5 min, then in acetone at -20C for 30 sec. After 3 washes in PBS, 0.1% BSA, were incubated for 1 hr at room temperature with 20 ug/ml of biotinylated H11 antibody in PBS, 0.1% BSA. After rinsing in PBS, FITC-conjugated streptavidin was added for 30 min, slides washed again in PBS and visualized using a fluorescence microscope.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_5/13893_Fas-Ligand-Antibody-%2528H11%2529-%255BBiotin%255D-Immunocytochemistry-Immunofluorescence-NBP1-97621-img0002.jpg)
Immunocytochemistry/Immunofluorescence: Fas Ligand Antibody (H11) [Biotin] [NBP1-97621] - Detection of FasL in 293T cells transfected with a FasL expression vector. Method: Transfected cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20C for 5 min, then in acetone at -20C for 30 sec. After 3 washes in PBS, 0.1% BSA, were incubated for 1 hr at room temperature with 20 ug/ml of biotinylated H11 antibody in PBS, 0.1% BSA. After rinsing in PBS, FITC-conjugated streptavidin was added for 30 min, slides washed again in PBS and visualized using a fluorescence microscope.
Product References
Characterization of the non-functional Fas ligand of gld mice. - Characterization of the non-functional Fas ligand of gld mice.
Hahne M, Peitsch MC, Irmler M, Schroter M, Lowin B, Rousseau M, Bron C, Renno T, French L, Tschopp J. Int Immunol. 1995 Sep;7(9):1381-6.
CD4+ T cells reactivated with superantigen are both more sensitive to - CD4+ T cells reactivated with superantigen are both more sensitive to
Wang JK, Zhu B, Ju ST, Tschopp J, Marshak-Rothstein A. Cell Immunol. 1997 Aug 1;179(2):153-64.
Transgenic expression of CD95 ligand on thyroid follicular cells confers immune - Transgenic expression of CD95 ligand on thyroid follicular cells confers immune
Tourneur L, Malassagne B, Batteux F, Fabre M, Mistou S, Lallemand E, Lores P, Chiocchia G. J Immunol. 2001 Aug 1;167(3):1338-46.