Phospho-Rpb1 CTD (Ser2/Ser5) Antibody (IP Preferred)

• Supplier: Cell Signaling Technology
• Catalog: 8860
• Host: Rabbit
• Clonality: Polyclonal
• Antigen: Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser2/Ser5 of the Rpb1 CTD heptapeptide repeat
• Description: Rabbit Polyclonal
• Gene: POLR2A
• Conjugate: IP Preferred

Product images

Western blot analysis of extracts from various cell lines using Phospho-Rpb1 CTD (Ser2/Ser5) Antibody (IP Preferred).

Immunoprecipitation of Phospho-Rpb1 CTD (Ser2/Ser5) from HeLa cell extracts, using Normal Rabbit IgG #2729 (lane 2) or Phospho-Rpb1 CTD (Ser2/Ser5) Antibody (IP Preferred) (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-Rpb1 CTD (Ser2/Ser5) Antibody (IP Preferred).

Phospho-Rpb1 CTD antibody specificity is demonstrated using peptide dot blot. Antibody binding to pre-coated Rpb1 CTD peptides is shown using Phospho-Rpb1 CTD (Ser2) Antibody #8798, Phospho-Rpb1 CTD (Ser5) Antibody #8807, a phospho-Rpb1 CTD (Ser7) antibody, and Phospho-Rpb1 CTD (Ser2/Ser5) Antibody (IP Preferred) #8860. As expected, the Phospho-Rpb1 CTD (Ser2/Ser5) Antibody (IP Preferred) #8860 only binds to the phospho-Rpb1 CTD peptide that is dually phosphorylated at Ser2 and Ser5.