Paraformaldehyde-fixed, paraffin embedded rat muscle tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-CACNA1F Polyclonal Antibody, Unconjugated (bs-11245R) at 1:300 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat muscle tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-CACNA1F Polyclonal Antibody, Unconjugated (bs-11245R) at 1:400 overnight at 4°C, followed by a biotin conjugated secondary for 20 minutes and Cy3 streptavidin at 1:500 for 40 minutes and DAPI staining