Formalin-fixed and paraffin embedded mouse heart tissue labeled with Anti-kir 6.1/IRK8 Polyclonal Antibody, Unconjugated (bs-6468R) at 1:200 followed by conjugation to the secondary antibody
Lane 1: mouse brain lysates Lane 2: mouse heart lysates probed with Anti kir 6.1/IRK8 Polyclonal Antibody, Unconjugated (bs-6468R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 47kD. Observed band size: 47kD.
H9C2 cells probed with Rabbit Anti-kir 6.1 Polyclonal Antibody, Unconjugated (bs-6468R) at 3ug for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-FITC) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).