Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti CDCA5/Sororin Polyclonal Antibody, Unconjugated (bs-7717R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded human rectal carcinoma labeled with Anti-CDCA5/Sororin Polyclonal Antibody, Unconjugated(bs-7717R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
Antigen: bs-7717P, 0.2ug/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Lane 1: mouse brain lysates Lane 2: human colon carcinoma lysates probed with Anti CDCA5/Sororin Polyclonal Antibody, Unconjugated (bs-7717R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 28kD. Observed band size: 28kD.