Paraformaldehyde-fixed, paraffin embedded Mouse heart tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RNF169 Polyclonal Antibody, Unconjugated (bs-9259R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse heart tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RNF169 Polyclonal Antibody, Unconjugated (bs-9259R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAPI staining
Paraformaldehyde-fixed, paraffin embedded human cervical cancer tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RNF169 Polyclonal Antibody, Unconjugated (bs-9259R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining