Mouse spleen lysate probed with Rabbit Anti-Secretogranin-5 Polyclonal Antibody, Unconjugated (bs-10762R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded rat adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Secretogranin V Polyclonal Antibody, Unconjugated (bs-10762)t 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.