Western blot analysis of human recombinant Bad fusion protein (A,B). Antibodies tested include Bad (H-168): sc-7869 (A) and Bad (N-19): sc-6542 (B).
Bad siRNA (h): sc-29778. Immunofluorescence staining of methanol-fixed, control HeLa (A) and Bad siRNA silenced HeLa (B) cells showing diminished cytoplasmic staining in the siRNA silenced cells. Cells probed with Bad (H-168): sc-7869.
Bad (H-168): sc-7869. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Bad (H-168): sc-7869. Western blot analysis of Bad expression in non-transfected: sc-117752 (A) and human Bad transfected: sc-170552 (B) 293T whole cell lysates.
Western blot analysis of Bad phosphorylation in non-transfected: sc-117752 (A,E), untreated human Bad transfected: sc-170552 (B,F), PMA treated human Bad transfected: sc-170552 (C,G) and PMA and lambda protein phosphatase (sc-200312A) treated human Bad transfected: sc-170552 (D,H) 293T whole cell lysates.Antibodies tested include p-Bad (C-10): sc-166932 (A,B,C,D) and Bad (H-168): sc-7869 (E,F,G,H).
Western blot analysis of Bad phosphorylation in non-transfected: sc-117752 (A,E), untreated human Bad transfected: sc-170552 (B,F), PMA treated human Bad transfected: sc-170552 (C,G) and PMA and lambda protein phosphatase (sc-200312A) treated human Bad transfected: sc-170552 (D,H) 293T whole cell lysates.Antibodies tested include p-Bad (Ser 112)-R: sc-7998-R (A,B,C,D) and Bad (H-168): sc-7869 (E,F,G,H).
Western blot analysis of Bad phosphorylation in non-transfected: sc-117752 (A,E), untreated human Bad transfected: sc-170552 (B,F), PMA treated human Bad transfected: sc-170552 (C,G) and PMA and lambda protein phosphatase treated human Bad transfected: sc-170552 (D,H) 293T whole cell lysates. Antibodies tested include p-Bad (C-10): sc-166932 (A,B,C) and Bad (H-168): sc-7869 (D,E,F).
Bad (H-168): sc-7869. Western blot analysis of Bad expression in BJAB (A) and MCF7 (B) whole cell lysates and mouse prostate (C) and rat prostate (D) tissue extracts.