Monoclonal Anti-WASH1 antibody produced in mouse

Name	Monoclonal Anti-WASH1 antibody produced in mouse
Supplier	Sigma-Aldrich
Catalog	SAB4200552
Prices	$337.00
Sizes	200 µl
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone	WASH1-27
Applications	IP WB
Species Reactivities	Human, Mouse
Antigen	a synthetic peptide corresponding to an internal region of human WASH1 (GeneID: 100287171), conjugated to KLH
Description	Mouse Monoclonal
Gene	WASH1
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>WT MEF cells (WASH +/+) or WASHout MEF cells (WASH-/-) were fixed in 4% PFA for 20 minutes and permeabilized with 0.15% Surfactamps for 3 minutes. Fixed cells were stained with 1:300 dilution of Monoclonal Anti-WASH1 Clone: WASH1-27 (<B>Cat. No. SAB4200552</B>). The antibody was developed using Goat Anti-Mouse IgG, TRITC conjugate. Cells were counterstained with Hoechst (blue) to stain nuclei. Pictures are courtesy of Timothy S. Gomez and Daniel D. Billadeau, Departments of Biochemistry and Molecular Biology and Immunology, Division of Oncology Research, Mayo Clinic, Rochester, USA.

Immunofluorescence
WT MEF cells (WASH +/+) or WASHout MEF cells (WASH-/-) were fixed in 4% PFA for 20 minutes and permeabilized with 0.15% Surfactamps for 3 minutes. Fixed cells were stained with 1:300 dilution of Monoclonal Anti-WASH1 Clone: WASH1-27 (Cat. No. SAB4200552). The antibody was developed using Goat Anti-Mouse IgG, TRITC conjugate. Cells were counterstained with Hoechst (blue) to stain nuclei. Pictures are courtesy of Timothy S. Gomez and Daniel D. Billadeau, Departments of Biochemistry and Molecular Biology and Immunology, Division of Oncology Research, Mayo Clinic, Rochester, USA.

<B>Immunoblotting</B><BR/>Whole extract of HEK-293T cells overexpressing mouse WASH1 was separated on SDS-PAGE and probed with Monoclonal Anti-WASH1 Clone: WASH1-27 (<B>Cat. No. SAB4200552</B>). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (<B>Cat. No. A2304</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. 2 μg/mL antibody<BR/>2. 5 μg/mL antibody<BR/>3. 5 μg/mL antibody + WASH1 immunizing peptide

Immunoblotting
Whole extract of HEK-293T cells overexpressing mouse WASH1 was separated on SDS-PAGE and probed with Monoclonal Anti-WASH1 Clone: WASH1-27 (Cat. No. SAB4200552). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
Lanes
1. 2 μg/mL antibody
2. 5 μg/mL antibody
3. 5 μg/mL antibody + WASH1 immunizing peptide

<B>Immunoblotting</B><BR/>Whole extracts of WT MEF cells (WASH +/+) or WASHout MEF cells (WASH-/-) were separated on SDS-PAGE and probed with 1:1,000 dilution of Monoclonal Anti-WASH1 Clone: WASH1-27 (<B>Cat. No. SAB4200552</B>). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. The picture is courtesy of Timothy S. Gomez and Daniel D. Billadeau, Departments of Biochemistry and Molecular Biology and Immunology, Division of Oncology Research, Mayo Clinic, Rochester, USA.

Immunoblotting
Whole extracts of WT MEF cells (WASH +/+) or WASHout MEF cells (WASH-/-) were separated on SDS-PAGE and probed with 1:1,000 dilution of Monoclonal Anti-WASH1 Clone: WASH1-27 (Cat. No. SAB4200552). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. The picture is courtesy of Timothy S. Gomez and Daniel D. Billadeau, Departments of Biochemistry and Molecular Biology and Immunology, Division of Oncology Research, Mayo Clinic, Rochester, USA.

<B>Immunoprecipitation</B><BR/>Monoclonal Anti-WASH1 Clone: WASH1-27 (<B>Cat. No. SAB4200552</B>) was used to immunoprecipitate WASH1 from HeLa whole cell extract. Detection antibody: Rabbit Anti-WASH1 (<B>Cat. No. SAB4200372</B>).<BR/><B>Lanes</B><BR/>1. 5 μg antibody<BR/>2. 10 μg antibody<BR/>3. Negative control: 10 μg non-relevant antibody

Immunoprecipitation
Monoclonal Anti-WASH1 Clone: WASH1-27 (Cat. No. SAB4200552) was used to immunoprecipitate WASH1 from HeLa whole cell extract. Detection antibody: Rabbit Anti-WASH1 (Cat. No. SAB4200372).
Lanes
1. 5 μg antibody
2. 10 μg antibody
3. Negative control: 10 μg non-relevant antibody