Anti-FOLR2 (81-95) antibody produced in rabbit

Name	Anti-FOLR2 (81-95) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	F2682
Prices	$328.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 81-95 of human FOLR2
Description	Rabbit Polyclonal
Gene	FOLR2
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human FLAG-FOLR2 fusion protein was separated on SDS-PAGE, blotted with Anti-FOLR2 (81-95) (<B>Cat. No. F2682</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:250 <BR/>2. Negative control (only secondary antibody)<BR/>3. Anti-FLAG antibody (<B>Cat. No. F7425</B>), working concentration 2 μg/mL

Immunoblotting
Lysate of HEK-293T cells overexpressing human FLAG-FOLR2 fusion protein was separated on SDS-PAGE, blotted with Anti-FOLR2 (81-95) (Cat. No. F2682) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:250
2. Negative control (only secondary antibody)
3. Anti-FLAG antibody (Cat. No. F7425), working concentration 2 μg/mL

<B>Immunofluorescence</B><BR/>Anti-FOLR2 (81-95): <B>Cat. No. F2682</B>: Immunofluorescence of HUVEC cells using FOLR2 (81-95), Cat. No. F2682 (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunofluorescence
Anti-FOLR2 (81-95): Cat. No. F2682: Immunofluorescence of HUVEC cells using FOLR2 (81-95), Cat. No. F2682 (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

<B>Chart</B><BR/>Anti-FOLR2 (81-95): <B>Cat. No. F2682</B>: Intensity analysis of Anti-FOLR2 (81-95) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Chart
Anti-FOLR2 (81-95): Cat. No. F2682: Intensity analysis of Anti-FOLR2 (81-95) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Product References

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Selective expression of the high-affinity isoform of the folate receptor - Selective expression of the high-affinity isoform of the folate receptor

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UMSCC38 cells amplified at 11q13 for the folate receptor synthesize a mutant - UMSCC38 cells amplified at 11q13 for the folate receptor synthesize a mutant

Orr RB, Kamen BA. Cancer Res. 1994 Jul 15;54(14):3905-11.

Studies of the role of a particulate folate-binding protein in the uptake of - Studies of the role of a particulate folate-binding protein in the uptake of

Antony AC, Kane MA, Portillo RM, Elwood PC, Kolhouse JF. J Biol Chem. 1985 Dec 5;260(28):14911-7.

Homologous membrane folate binding proteins in human placenta: cloning and - Homologous membrane folate binding proteins in human placenta: cloning and

Ratnam M, Marquardt H, Duhring JL, Freisheim JH. Biochemistry. 1989 Oct 3;28(20):8249-54.

Structures of human folate receptors reveal biological trafficking states and - Structures of human folate receptors reveal biological trafficking states and

Wibowo AS, Singh M, Reeder KM, Carter JJ, Kovach AR, Meng W, Ratnam M, Zhang F, Dann CE 3rd. Proc Natl Acad Sci U S A. 2013 Sep 17;110(38):15180-8. doi: