Anti-HIP14 antibody produced in rabbit

• Supplier: Sigma-Aldrich
• Catalog: H7414
• Prices: $69.10, $392.50
• Sizes: 25 µl, 200 µl
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB
• Species Reactivities: Human, Mouse, Rat, Dog, Bovine
• Antigen: synthetic peptide corresponding to amino acids 614-632 located at the C-terminus of human HIP14
• Description: Rabbit Polyclonal
• Gene: ZDHHC17
• Conjugate: unconjugated

Product images

Immunoblotting
Whole cell lysates and brain extracts (P2 membrane fractions) were separated on SDS-PAGE and blotted with Anti-HIP14 (Cat. No. H7414). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution 1:4,000
Lanes
1: HEK-293T cells transfected with human HIP14-FLAG
2: Untransfected HEK-293T cells
3: HEK-293T cells transfected with human HIP14-FLAG + Inhibition with HIP14 (immunizing peptide human 614-632) (20 μg/mL)
4: Mouse brain (membrane P2 fraction)
5: Rat brain (membrane P2 fraction)

Product References

Wild-type HTT modulates the enzymatic activity of the neuronal palmitoyl - Wild-type HTT modulates the enzymatic activity of the neuronal palmitoyl

Huang K, Sanders SS, Kang R, Carroll JB, Sutton L, Wan J, Singaraja R, Young FB, Liu L, El-Husseini A, Davis NG, Hayden MR. Hum Mol Genet. 2011 Sep 1;20(17):3356-65.

Huntingtin-interacting protein 14 is a type 1 diabetes candidate protein - Huntingtin-interacting protein 14 is a type 1 diabetes candidate protein

Berchtold LA, Storling ZM, Ortis F, Lage K, Bang-Berthelsen C, Bergholdt R, Hald J, Brorsson CA, Eizirik DL, Pociot F, Brunak S, Storling J. Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):E681-8. doi: