Anti-EXOSC5 (N-terminal) antibody produced in rabbit

Name	Anti-EXOSC5 (N-terminal) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	SAB4200439
Prices	$337.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	IP ICC/IF WB
Species Reactivities	Human
Antigen	peptide corresponding to the N-terminal region of human EXOSC5, conjugated to KLH
Description	Rabbit Polyclonal
Gene	EXOSC5
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton<SUP>™</SUP> X-100. Fixed cells were stained with 2 μg/mL Rabbit Anti-EXOSC5 (N-Terminal) (<B>Cat. No. SAB4200439</B>). The antibody was developed using Goat Anti-Rabbit IgG, Cy3<SUP>™</SUP> conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

Immunofluorescence
HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton^™ X-100. Fixed cells were stained with 2 μg/mL Rabbit Anti-EXOSC5 (N-Terminal) (Cat. No. SAB4200439). The antibody was developed using Goat Anti-Rabbit IgG, Cy3^™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

<B>Immunoblotting</B><BR/>Whole extract of HEK-293T cells was separated on SDS-PAGE and probed with Rabbit Anti-EXOSC5 (N-terminal) (<B>Cat. No. SAB4200439</B>). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. 2 μg/mL antibody<BR/>2. 1 μg/mL antibody<BR/>3. 2 μg/mL antibody + EXOSC5 immunizing peptide

Immunoblotting
Whole extract of HEK-293T cells was separated on SDS-PAGE and probed with Rabbit Anti-EXOSC5 (N-terminal) (Cat. No. SAB4200439). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. 2 μg/mL antibody
2. 1 μg/mL antibody
3. 2 μg/mL antibody + EXOSC5 immunizing peptide

<B>Immunoprecipitation</B><BR/>Rabbit Anti-EXOSC5 (N-Terminal) (<B>Cat. No. SAB4200439</B>) was used to immunoprecipitate EXOSC5 from HeLa whole cell extract.<BR/>Detection antibody: Rabbit Anti-EXOSC5 (C-Terminal) (<B>Cat. No. SAB4200438</B>).<BR/><B>Lanes</B><BR/>1. 10 μg antibody <BR/>2. 5 μg antibody<BR/>3. 2.5 μg antibody<BR/>4. Negative control: non-relevant antibody<BR/>5. Negative control: without 1st antibody<BR/>6. Negative control: without cell extract

Immunoprecipitation
Rabbit Anti-EXOSC5 (N-Terminal) (Cat. No. SAB4200439) was used to immunoprecipitate EXOSC5 from HeLa whole cell extract.
Detection antibody: Rabbit Anti-EXOSC5 (C-Terminal) (Cat. No. SAB4200438).
Lanes
1. 10 μg antibody
2. 5 μg antibody
3. 2.5 μg antibody
4. Negative control: non-relevant antibody
5. Negative control: without 1st antibody
6. Negative control: without cell extract