Anti-SND1 antibody produced in rabbit

Name	Anti-SND1 antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	SAB4200470
Prices	$337.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	IP ICC/IF WB
Species Reactivities	Rat, Human, Mouse
Antigen	peptide corresponding to an internal region of human SND1, conjugated to KLH
Description	Rabbit Polyclonal
Gene	SND1
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunoblotting</B><BR/>Cell line lysates were separated on SDS-PAGE and probed with 0.5 μg/mL Rabbit Anti-SND1 (<B>Cat. No. SAB4200470</B>). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/>Lanes<BR/>1. HeLa<BR/>2. HEK-293T<BR/>3. NRK<BR/>4. RAT2<BR/>5. 3T3<BR/>6. Hepa1-6<BR/>7. MBCK

Immunoblotting
Cell line lysates were separated on SDS-PAGE and probed with 0.5 μg/mL Rabbit Anti-SND1 (Cat. No. SAB4200470). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. HeLa
2. HEK-293T
3. NRK
4. RAT2
5. 3T3
6. Hepa1-6
7. MBCK

<B>Immunofluorescence</B><BR/>HeLa cells were fixed and permeabilized with cold methanol followed by cold acetone. Fixed cells were stained with 2.5 μg/mL Rabbit Anti-SND1 (<B>Cat. No. SAB4200470</B>). The antibody was developed using Goat Anti-Rabbit IgG, Atto488 conjugate (<B>Cat. No. 18772</B>).

Immunofluorescence
HeLa cells were fixed and permeabilized with cold methanol followed by cold acetone. Fixed cells were stained with 2.5 μg/mL Rabbit Anti-SND1 (Cat. No. SAB4200470). The antibody was developed using Goat Anti-Rabbit IgG, Atto488 conjugate (Cat. No. 18772).

<B>Immunoprecipitation</B><BR/>Rabbit Anti-SND1 (<B>Cat. No. SAB4200470</B>) was used to immunoprecipitate SND1 from NRK whole cell extract. Detection antibody: Rabbit Anti-SND1 (C-terminal) (<B>Cat. No. SAB4200471</B>).<BR/>Lanes<BR/>1. 1 μg antibody<BR/>2. 2 μg antibody<BR/>3. Negative control: 2 μg non-relevant antibody<BR/>4. Positive control: NRK whole cell extract

Immunoprecipitation
Rabbit Anti-SND1 (Cat. No. SAB4200470) was used to immunoprecipitate SND1 from NRK whole cell extract. Detection antibody: Rabbit Anti-SND1 (C-terminal) (Cat. No. SAB4200471).
Lanes
1. 1 μg antibody
2. 2 μg antibody
3. Negative control: 2 μg non-relevant antibody
4. Positive control: NRK whole cell extract