Anti-TNPO2 (N-terminal) antibody produced in rabbit

Name	Anti-TNPO2 (N-terminal) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	SAB4200032
Prices	$61.10, $360.00
Sizes	25 µl, 200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	IP ICC/IF WB
Antigen	synthetic peptide corresponding to amino acids 19-34 of human TNPO2 conjugated to KLH
Description	Rabbit Polyclonal
Gene	TNPO2
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>Predominant nuclear localization of TNPO2 in HEK-293T cells. Cells were fixed and stained with 5 μg/mL Rabbit Anti-TNPO2 (N-terminal) (<B>Cat. No. SAB4200032</B>) followed by Goat Anti-Rabbit, Cy3 conjugated (×40 magnification).

Immunofluorescence
Predominant nuclear localization of TNPO2 in HEK-293T cells. Cells were fixed and stained with 5 μg/mL Rabbit Anti-TNPO2 (N-terminal) (Cat. No. SAB4200032) followed by Goat Anti-Rabbit, Cy3 conjugated (×40 magnification).

<B>Immunoblotting</B><BR/>Lysates of various cell lines were separated on SDS-PAGE and probed with 4 μg/mL Rabbit Anti-TNPO2 (N-terminal) (<B>Cat. No. SAB4200032</B>). The antibody was developed with Goat Anti-Rabbit IgG (<B>Cat. No. A0545</B>) Peroxidase conjugate and a chemiluminescence substrate. <BR/><B>Lanes</B><BR/>1. HEK-293T cells; 2. Jurkat; 3. HeLa; 4. K562; 5. MCF7

Immunoblotting
Lysates of various cell lines were separated on SDS-PAGE and probed with 4 μg/mL Rabbit Anti-TNPO2 (N-terminal) (Cat. No. SAB4200032). The antibody was developed with Goat Anti-Rabbit IgG (Cat. No. A0545) Peroxidase conjugate and a chemiluminescence substrate.
Lanes
1. HEK-293T cells; 2. Jurkat; 3. HeLa; 4. K562; 5. MCF7

<B>Immunoprecipitation</B><BR/>Rabbit Anti-TNPO2 (N-terminal) (<B>Cat. No. SAB4200031</B>) and Rabbit Anti-TNPO2 (C-terminal) (<B>Cat. No. SAB4200032</B>) were used to immunoprecipitate human TNPO2 from lysate of HEK-293T cells over-expressing human TNPO2. <BR/><B>Lanes</B><BR/>1. IP antibody: 10 μg Anti-TNPO2 (N-terminal) (<B>Cat. No. SAB4200032</B>) <BR/>2. IP antibody: 10 μg Anti-TNPO2 (C-terminal) (<B>Cat. No. SAB4200031</B>)<BR/>3. Negative control: Anti-TNPO2 (N-terminal) IP without cell lysate<BR/>4. Negative control: Anti-TNPO2 (C-terminal) IP without cell lysate <BR/>5. Negative control: Anti-TNPO2 (N-terminal) IP without IP antibody<BR/>6. Negative control: Anti-TNPO2 (C-terminal) IP without IP antibody<BR/>7. Immunoblotting positive control: total HEK-293T cells over-expressing human TNPO2 blot with Anti-TNPO2 (N-terminal) (<B>Cat. No. SAB4200032</B>) <BR/>8. Immunoblotting positive control: total HEK-293T cells over-expressing human TNPO2 blot with Anti-TNPO2 (C-terminal) (

Immunoprecipitation
Rabbit Anti-TNPO2 (N-terminal) (Cat. No. SAB4200031) and Rabbit Anti-TNPO2 (C-terminal) (Cat. No. SAB4200032) were used to immunoprecipitate human TNPO2 from lysate of HEK-293T cells over-expressing human TNPO2.
Lanes
1. IP antibody: 10 μg Anti-TNPO2 (N-terminal) (Cat. No. SAB4200032)
2. IP antibody: 10 μg Anti-TNPO2 (C-terminal) (Cat. No. SAB4200031)
3. Negative control: Anti-TNPO2 (N-terminal) IP without cell lysate
4. Negative control: Anti-TNPO2 (C-terminal) IP without cell lysate
5. Negative control: Anti-TNPO2 (N-terminal) IP without IP antibody
6. Negative control: Anti-TNPO2 (C-terminal) IP without IP antibody
7. Immunoblotting positive control: total HEK-293T cells over-expressing human TNPO2 blot with Anti-TNPO2 (N-terminal) (Cat. No. SAB4200032)
8. Immunoblotting positive control: total HEK-293T cells over-expressing human TNPO2 blot with Anti-TNPO2 (C-terminal) (