Anti-EXOSC5 (C-terminal) antibody produced in rabbit

Name	Anti-EXOSC5 (C-terminal) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	SAB4200438
Prices	$337.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	IP ICC/IF WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to the C-terminal region of human EXOSC5, conjugated to KLH
Description	Rabbit Polyclonal
Gene	EXOSC5
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton<SUP>™</SUP> X-100. Fixed cells were stained with 5 μg/mL Rabbit Anti-EXOSC5 (C-Terminal) (<B>Cat. No. SAB4200438</B>). The antibody was developed using Goat Anti-Rabbit IgG, Cy3<SUP>™</SUP> conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

Immunofluorescence
HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton^™ X-100. Fixed cells were stained with 5 μg/mL Rabbit Anti-EXOSC5 (C-Terminal) (Cat. No. SAB4200438). The antibody was developed using Goat Anti-Rabbit IgG, Cy3^™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

<B>Immunoprecipitation</B><BR/>Rabbit Anti-EXOSC5 (C-Terminal) (<B>Cat. No. SAB4200438</B>) was used to immunoprecipitate EXOSC5 from HeLa whole cell extract. Detection antibody: Rabbit Anti-EXOSC5 (N-Terminal) (<B>Cat. No. SAB4200439</B>).<BR/><B>Lanes</B><BR/>1. 10 μg antibody <BR/>2. 5 μg antibody<BR/>3. 2.5 μg antibody<BR/>4. 1.2 μg antibody<BR/>5. Negative control: non-relevant antibody<BR/>6. Negative control: without cell extract<BR/>7. Negative control: without 1st antibody

Immunoprecipitation
Rabbit Anti-EXOSC5 (C-Terminal) (Cat. No. SAB4200438) was used to immunoprecipitate EXOSC5 from HeLa whole cell extract. Detection antibody: Rabbit Anti-EXOSC5 (N-Terminal) (Cat. No. SAB4200439).
Lanes
1. 10 μg antibody
2. 5 μg antibody
3. 2.5 μg antibody
4. 1.2 μg antibody
5. Negative control: non-relevant antibody
6. Negative control: without cell extract
7. Negative control: without 1st antibody