Anti-ANAPC2 (203-215) antibody produced in rabbit

• Supplier: Sigma-Aldrich
• Catalog: A4358
• Prices: $342.00
• Sizes: 200 µl
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB
• Species Reactivities: Human
• Antigen: synthetic peptide corresponding to amino acids 203-215 of human ANAPC2
• Description: Rabbit Polyclonal
• Gene: ANAPC2
• Conjugate: unconjugated

Product images

Immunofluorescence
Anti-ANAPC2 (203-215): Cat. No. A4358: Immunofluorescence of HUVEC cells using ANAPC2 (203-215) (RB),Cat. No. A4358 (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunoblotting
Lysate of HEK-293T cells overexpressing human ANAPC2 was separated on SDS-PAGE, blotted with Anti-ANAPC2 (203-215) (Cat. No. A4358) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:1,000

Chart
Anti-ANAPC2 (203-215): Cat. No. A4358: Intensity analysis of Anti-ANAPC2 (203-215) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Product References

FBG1 is a promiscuous ubiquitin ligase that sequesters APC2 and causes S-phase - FBG1 is a promiscuous ubiquitin ligase that sequesters APC2 and causes S-phase

Wen H, Kim N, Fuentes EJ, Mallinger A, Gonzalez-Alegre P, Glenn KA. Cell Cycle. 2010 Nov 15;9(22):4506-17.

APC2 Cullin protein and APC11 RING protein comprise the minimal ubiquitin ligase - APC2 Cullin protein and APC11 RING protein comprise the minimal ubiquitin ligase

Tang Z, Li B, Bharadwaj R, Zhu H, Ozkan E, Hakala K, Deisenhofer J, Yu H. Mol Biol Cell. 2001 Dec;12(12):3839-51.