Anti-DLC1 antibody produced in rabbit

Name	Anti-DLC1 antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	SAB4200351
Prices	$342.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	ICC/IF IP WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to a sequence near the N-terminal region of human DLC1, conjugated to KLH
Description	Rabbit Polyclonal
Gene	DLC1
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B> <BR/>Cytoplasmic and perinuclear localization of DLC1 in HEK293-T cells overexpressing human DLC1. Fixed cells were stained with 1 μg/mL Anti-DLC1 (<B>Cat. No. SAB4200351</B>) followed by Goat Anti-Rabbit IgG, Cy3 conjugate (×40 magnification). Nuclear staining with DAPI.

Immunofluorescence
Cytoplasmic and perinuclear localization of DLC1 in HEK293-T cells overexpressing human DLC1. Fixed cells were stained with 1 μg/mL Anti-DLC1 (Cat. No. SAB4200351) followed by Goat Anti-Rabbit IgG, Cy3 conjugate (×40 magnification). Nuclear staining with DAPI.

<B>Immunoblotting</B><BR/>Cell lysates were separated on SDS-PAGE, blotted with 0.5 μg/mL Anti-DLC1 (<B>Cat. No. SAB4200351</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. HEK293-T overexpressing human DLC1<BR/>2. HEK293-T non-transfected<BR/>3. HEK293-T overexpressing human DLC1 + immunizing DLC1 peptide.

Immunoblotting
Cell lysates were separated on SDS-PAGE, blotted with 0.5 μg/mL Anti-DLC1 (Cat. No. SAB4200351) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. HEK293-T overexpressing human DLC1
2. HEK293-T non-transfected
3. HEK293-T overexpressing human DLC1 + immunizing DLC1 peptide.

<B>Immunoprecipitation</B><BR/>Anti-DLC1 (<B>Cat. No. SAB4200351</B>), was used to immunoprecipitate DLC1 from HEK293-T cells overexpressing human DLC1. Detection antibody: Anti-DLC1 (C-terminal) (<B>Cat. No. SAB4200352</B>).<BR/><B>Lanes</B><BR/>1. IP antibody: 10 μg<BR/>2. Negative control: without cell lysate<BR/>3. Negative control: without IP antibody

Immunoprecipitation
Anti-DLC1 (Cat. No. SAB4200351), was used to immunoprecipitate DLC1 from HEK293-T cells overexpressing human DLC1. Detection antibody: Anti-DLC1 (C-terminal) (Cat. No. SAB4200352).
Lanes
1. IP antibody: 10 μg
2. Negative control: without cell lysate
3. Negative control: without IP antibody