Anti-CYP51A1 (495-509) antibody produced in rabbit

• Supplier: Sigma-Aldrich
• Catalog: C8873
• Prices: $309.50
• Sizes: 200 µl
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB
• Species Reactivities: Human
• Antigen: synthetic peptide corresponding to amino acids 495-509 of human CYP51A1
• Description: Rabbit Polyclonal
• Gene: CYP51A1
• Conjugate: unconjugated

Product images

Immunofluorescence
Anti-CYP51A1 (495-509): Cat. No. C8873: Immunofluorescence of HUVEC cells using CYP51A1 (495-509), Cat. No. C8873 (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunoblotting
HEK-293T cell lysate was separated on SDS-PAGE, blotted with Anti-CYP51A1 (495-509) (Cat. No. C8873) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:500

Chart
Anti-CYP51A1 (495-509): Cat. No. C8873: Intensity analysis of Anti-CYP51A1 (495-509) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Product References

Structural basis of human CYP51 inhibition by antifungal azoles. - Structural basis of human CYP51 inhibition by antifungal azoles.

Strushkevich N, Usanov SA, Park HW. J Mol Biol. 2010 Apr 9;397(4):1067-78.

Crystal structure of cytochrome P450 14alpha -sterol demethylase (CYP51) from - Crystal structure of cytochrome P450 14alpha -sterol demethylase (CYP51) from

Podust LM, Poulos TL, Waterman MR. Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3068-73.