Anti-Rab13 (C-terminal) antibody produced in rabbit

Name	Anti-Rab13 (C-terminal) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	SAB4200058
Prices	$61.10, $360.00
Sizes	25 µl, 200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	IP ICC/IF WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 183-198 of human Rab13, conjugated to KLH
Description	Rabbit Polyclonal
Gene	RAB13
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>A431 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton<SUP>™</SUP> X-100. Fixed cells were stained with 5 μg/mL Rabbit Anti-Rab13 (C-terminal) (<B>Cat. No. SAB4200058</B>). The antibody was developed using Goat Anti-Rabbit IgG, Atto 488 conjugate (<B>Cat. No. 18772</B>).Cells were counterstained with DAPI (blue) to stain nuclei (×100 magnification).

Immunofluorescence
A431 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton^™ X-100. Fixed cells were stained with 5 μg/mL Rabbit Anti-Rab13 (C-terminal) (Cat. No. SAB4200058). The antibody was developed using Goat Anti-Rabbit IgG, Atto 488 conjugate (Cat. No. 18772).Cells were counterstained with DAPI (blue) to stain nuclei (×100 magnification).

<B>Immunofluorescence</B><BR/>NIH-3T3 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton<SUP>™</SUP> X-100. Fixed cells were stained with 5 μg/mL Rabbit Anti-Rab13 (C-terminal) (<B>Cat. No. SAB4200058</B>). The antibody was developed using Goat Anti-Rabbit IgG, Atto 488 conjugate (<B>Cat. No. 18772</B>).Cells were counterstained with DAPI (blue) to stain nuclei (×100 magnification).

Immunofluorescence
NIH-3T3 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton^™ X-100. Fixed cells were stained with 5 μg/mL Rabbit Anti-Rab13 (C-terminal) (Cat. No. SAB4200058). The antibody was developed using Goat Anti-Rabbit IgG, Atto 488 conjugate (Cat. No. 18772).Cells were counterstained with DAPI (blue) to stain nuclei (×100 magnification).

<B>Immunoblotting</B><BR/>Whole extract of NT2 cells was separated on SDS-PAGE and probed with Rabbit Anti-Rab13 (C-terminal) (<B>Cat. No. SAB4200058</B>). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. 2 μg/mL antibody<BR/>2. 5 μg/mL antibody<BR/>3. 5 μg/mL antibody + Rab13 immunizing peptide (human, 183-198)

Immunoblotting
Whole extract of NT2 cells was separated on SDS-PAGE and probed with Rabbit Anti-Rab13 (C-terminal) (Cat. No. SAB4200058). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. 2 μg/mL antibody
2. 5 μg/mL antibody
3. 5 μg/mL antibody + Rab13 immunizing peptide (human, 183-198)

<B>Immunoprecipitation</B><BR/>Rabbit Anti-Rab13 (C-terminal) (<B>Cat. No. SAB4200058</B>), was used to immunoprecipitate Rab13 from NT2 whole cell extract. Detection antibody: Rabbit Anti-Rab13.<BR/><B>Lanes</B><BR/>1. 5 μg antibody <BR/>2. 10 μg antibody <BR/>3. Negative control: 10 μg non-relevant antibody<BR/>4. Positive control: NT2 whole cell extract

Immunoprecipitation
Rabbit Anti-Rab13 (C-terminal) (Cat. No. SAB4200058), was used to immunoprecipitate Rab13 from NT2 whole cell extract. Detection antibody: Rabbit Anti-Rab13.
Lanes
1. 5 μg antibody
2. 10 μg antibody
3. Negative control: 10 μg non-relevant antibody
4. Positive control: NT2 whole cell extract