Anti-ALOX5AP (31-45) antibody produced in rabbit

Name	Anti-ALOX5AP (31-45) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	A0735
Prices	$328.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 31-45 of human ALOX5AP
Description	Rabbit Polyclonal
Gene	ALOX5AP
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>Anti-ALOX5AP (31-45): <B>Cat. No. A0735</B>: Immunofluorescence of HUVEC cells using ALOX5AP (31-45), Cat. No. A0735 (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunofluorescence
Anti-ALOX5AP (31-45): Cat. No. A0735: Immunofluorescence of HUVEC cells using ALOX5AP (31-45), Cat. No. A0735 (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human ALOX5AP was separated on SDS-PAGE, blotted with Anti-ALOX5AP (31-45) (<B>Cat. No. A0735</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:250

Immunoblotting
Lysate of HEK-293T cells overexpressing human ALOX5AP was separated on SDS-PAGE, blotted with Anti-ALOX5AP (31-45) (Cat. No. A0735) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:250

<B>Chart</B><BR/>Anti-ALOX5AP (31-45): <B>Cat. No. A0735</B>: Intensity analysis of Anti-ALOX5AP (31-45) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Chart
Anti-ALOX5AP (31-45): Cat. No. A0735: Intensity analysis of Anti-ALOX5AP (31-45) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Product References

The 5-lipoxygenase/leukotriene pathway in preclinical models of cardiovascular - The 5-lipoxygenase/leukotriene pathway in preclinical models of cardiovascular

Poeckel D, Funk CD. Cardiovasc Res. 2010 May 1;86(2):243-53.

The role of LTA4H and ALOX5AP polymorphism in asthma and allergy susceptibility. - The role of LTA4H and ALOX5AP polymorphism in asthma and allergy susceptibility.

Holloway JW, Barton SJ, Holgate ST, Rose-Zerilli MJ, Sayers I. Allergy. 2008 Aug;63(8):1046-53.

Association of a genetic variant in the ALOX5AP with higher risk of ischemic - Association of a genetic variant in the ALOX5AP with higher risk of ischemic

Domingues-Montanari S, Fernandez-Cadenas I, del Rio-Espinola A, Corbeto N, Krug T, Manso H, Gouveia L, Sobral J, Mendioroz M, Fernandez-Morales J, Alvarez-Sabin J, Ribo M, Rubiera M, Obach V, Marti-Fabregas J, Freijo M, Serena J, Ferro JM, Vicente AM, Oliveira SA, Montaner J. Cerebrovasc Dis. 2010;29(6):528-37.

Correlation between cerebral infarction and ALOX5AP gene expression. - Correlation between cerebral infarction and ALOX5AP gene expression.

Xu C, Qiang L, Limei C, Yan Z, Jia Z, Zhiqiang D, Zhao Z. Cell Biochem Biophys. 2013;67(3):899-904.